Regulator of G protein signaling (RGS) proteins terminate G protein signaling by enhancing the rate of GTP hydrolysis by the G\u03b1 subunit of G proteins. More than 20 different RGS proteins have been identified. Transcripts encoding most of them are present in the heart, but it is unclear how many different RGS proteins are expressed in the heart at the protein level. RGS proteins differ with respect to their tissue distribution and the specific G proteins that they interact with. This makes them attractive targets for the development of new therapeutic drugs.<\/p>\n\n\n\n
Our laboratory used a transgenic mouse model (developed by Richard Neubig) that expresses a form of G\u03b1i2 <\/a>that does not interact with RGS proteins. This model provides a tool to explore the impact of disrupting interactions between RGS proteins and G\u03b1i2. This enhances signaling through G\u03b1i2. G\u03b1i- coupled receptors have a well established role in cardioprotection. We found that disrupting the interaction between RGS proteins and G\u03b1i2 protects the heart from ischemic injury, suggesting that RGS proteins might provide a novel therapeutic target to protect the heart from myocardial ischemia.<\/p>\n\n\n\n We have used a variety of RGS knockout mouse lines to investigate the ability of specific RGS proteins to modulate the myocardial response to ischemic injury. Our work demonstrated that RGS6 protects the heart from ischemic injury.<\/p>\n\n\n\n Relevant Publications:<\/strong><\/p>\n\n\n\n Rorabaugh BR<\/strong>, Chakravarti B, Mabe NW, Seeley SL, Bui AD, Yang J, Watts SW, Neubig RR, Fisher RA (2017). Regulator of G Protein Signaling 6 Protects the Heart from Ischemic Injury. Journal of Pharmacology and Experimental Therapeutics <\/em>360:409-416. https:\/\/pubmed.ncbi.nlm.nih.gov\/28035008\/<\/a><\/p>\n\n\n\n Parra S, Huang X, Charbeneau R, Wade S, Kaur K, Rorabaugh BR<\/strong>, Neubig RR (2014) Conditional disruption of interactions between G\u03b1i2 and regulator of G protein signaling (RGS) proteins protects the heart from ischemic injury. BMC Pharmacology and Toxicology<\/em> 15:29 https:\/\/pubmed.ncbi.nlm.nih.gov\/24899231\/<\/a><\/p>\n\n\n\n Waterson R, Thompson C, Mabe N, Kaur K, Talbot JN, Neubig RR, Rorabaugh BR<\/strong> (2011) G\u03b1i2-mediated protection from ischemic injury is modulated by endogenous RGS proteins in the mouse heart. Cardiovascular Research<\/em> 91:45-52. https:\/\/pubmed.ncbi.nlm.nih.gov\/21349876\/<\/p>\n","protected":false},"excerpt":{"rendered":" Regulator of G protein signaling (RGS) proteins terminate G protein signaling by enhancing the rate of GTP hydrolysis by the G\u03b1 subunit of G proteins. More than 20 different RGS proteins have been identified. Transcripts encoding most of them are present in the heart, but it is unclear how many different RGS proteins are expressed […]<\/p>\n","protected":false},"author":70,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-47","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/mupages.marshall.edu\/rorabaughlab\/wp-json\/wp\/v2\/pages\/47","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mupages.marshall.edu\/rorabaughlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/mupages.marshall.edu\/rorabaughlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/mupages.marshall.edu\/rorabaughlab\/wp-json\/wp\/v2\/users\/70"}],"replies":[{"embeddable":true,"href":"https:\/\/mupages.marshall.edu\/rorabaughlab\/wp-json\/wp\/v2\/comments?post=47"}],"version-history":[{"count":3,"href":"https:\/\/mupages.marshall.edu\/rorabaughlab\/wp-json\/wp\/v2\/pages\/47\/revisions"}],"predecessor-version":[{"id":89,"href":"https:\/\/mupages.marshall.edu\/rorabaughlab\/wp-json\/wp\/v2\/pages\/47\/revisions\/89"}],"wp:attachment":[{"href":"https:\/\/mupages.marshall.edu\/rorabaughlab\/wp-json\/wp\/v2\/media?parent=47"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}